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KMID : 0613820110210060845
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Journal of Life Science
2011 Volume.21 No. 6 p.845 ~ p.850
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Study on Norovirus Genotypes in Busan, Korea
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Kim Nam-Ho
Park Eun-Hee
Park Yeon-Kyoung
Min Sang-Kee
Jin Seong-Hyeon
Park So-Hyun
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Abstract
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Norovirus (NoV) causes major acute non-bacterial gastroenteritis in humans. NoV genus is a member of the family Caliciviridae, which is transmitted by contaminated food and water or from human to human. Many genotypes of genogroups Ⅰ and Ⅱ have been reported because of their high genetic diversity. To obtain molecular epidemiological information on gastroenteritis sporadic cases in Busan, Korea, we analyzed the nucleotide sequences of NoV strains detected during 2008~2010. We performed one step RT-PCR amplifying the open reading frame (ORF) 2 (capsid region) followed by semi-nested PCR. Fecal samples were collected from 4,071 acute gastroenteritis, and genotypes of the 421 positive samples were determined by sequence analysis. Based on partial sequence of capsid region, 7 NoV were categorized into genogroup Ⅰ and 13 into genogroup Ⅱ. Prevalent genotypes among gastroenteritis patients within Busan were GII.4, GI.6, GII.5 in 2008~2010. The results of this study will contribute to the currently available epidemiological data and improve public health and hygiene via development of diagnostic methods and sustainable surveillance.
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KEYWORD
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Norovirus, gastroenteritis, genotype
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